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What happens in Chromatography?

What happens in Chromatography? Let us check it out how chromatography works and what is the principle behind chromatography. Principle of chromatography is given below.

  1. Chromatography is a method or technique used to separate or isolate different constituents of a solution mixture.
  2. Chromatography separates unknown sample from a give mixture.
  3. Compound which is to be separated is passed through chromatography column along with the mobile phase.
  4. There is stationary phase in the chromatography column.
  5. Now the molecules of given compouds interacts (adsorb) with the stationary phase.
  6. Molecules which have strong interaction will separate slower and molecules with weak interaction will move fast.
  7. The molecules of given compound must have solubility properties with the mobile phase.
  8. Finally molecules are detected at the detector and hence unknown molecule can be identified.

 

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Types of chromatography

There are various different types of chromatography which are used today in different industries and in day to day life.

Before going into detail let us check it out the definition of the chromatography.

The chromatogaphy is the separation of a component from of complex mixture of substance.

Types of component, functional group or structure can be identified with the help of chromatography.

List of some of the equipment are as follows:

Gas chromatography

Liquid chromatography

Solid chromatography

Liquid-liquid chromatography

Advantages and disadvantages of Chromatography

Chromatography is simple method. Chromatography can be handled by single person.

Other advantages are, Chromatography is used to separate components from complex mixture.

Different types of chromatography equipment are available for separation of different compounds.

Some of them are Gas Chromatography, Liquid Chromatography, Solid Chromatography, High performance liquid Chromatography, Solid liquid Chromatography, Liquid liquid Chromatography, Thin layer Chromatography. This method gives accurate results.

Full Form of NPC Chromatography

Normal Phase Chromatography (NPC) is an analytical method used to separate substance from a given mixture. (Other full form of NPC and related terms are also available) So check it out similar articles related to the given term.

Meaning and full form of other types of chromatography in chemistry are also available.

Full Form of TLC Chromatography

Thin Layer Chromatography (TLC) is an analytical method used to separate substance from a given mixture. (Other full form of TLC and related terms are also available) So check it out similar articles related to the given term.

Meaning and full form of other types of chromatography in chemistry are also available.